Waters Hplc System Cleaning Procedure

2 0 scope this procedure is applicable for of all high performance liquid chromatography installed in quality control laboratory.

Waters hplc system cleaning procedure.

Place a b c and d seal wash and needle wash lines into a clean. When preparing a system to run your method it is always important to prime up the system to ensure you get fresh solvents in all of the required lines. Flush the system using one of the cleaning mixtures as described in the cleaning solution table of the controlling contamination in uplc ms and hplc ms sytems guide 715001307. Technical assistant 3 2 checking.

To clean the system. Water flush to clear customer s mobile phases from the system. Reverse osmosis to remove most contaminants b. Disconnect the column and attach a union and flow directly to waste.

Remove all solvent filters. Cleaning mixtures for lc table in the controlling contamination in ultraperformance lc ms and hplc ms systems document p n 715001307. The water flushes are only needed to remove the previous solvent from the system. Water acetonitrile methanol ipa 1 1 1 1 with 2 5 fa.

This sop shall be applicable for the hplc system waters alliances 2690 2695 separation modules. No injections are needed with that. Controlling contamination in lc ms and hplc ms systems 715001307. Maintaining your hplc uhplc uplc systems.

To clearly lay down the procedure for cleaning. For information on other acquity uplc cleaning methods refer to the recommended cleaning mixtures for lc table in the con trolling contamination in ultraperformance lc ms and hplc ms systems document p n 715001307. Label bottles correctly with bottle content and filling date expiry date. 10 nh4oh in water this removes peg and other.

Cleaning decontamination columns general guidelines flow cells general. 3 0 responsibility 3 1 doing. Ion exchange to remove any remaining ions. Responsibility quality control officer executive.

To clean the system. It is necessary to restore the system flow path to a near new level of cleanliness note. Objective to lay down the procedure for operation of hplc waters alliance 2695 separation module scope to ensure that the instrument performs satisfactory and gives accurate and reproducible data. When working with any lc system setting it up and storing it is very important.

Use solvent inlet filters to protect the system from incoming particles. Use only hplc grade solvents and water filtered through 0 2. Water flush to clean acid from the system. Ultrapure water is water that has been purified through a system that targets contami nants detrimental to uplc ms and hplc ms systems.